Bioluminescent, Nonlytic, Real-Time Cell Viability Assay and Use in Inhibitor Screening.

ABSTRACT: Real-time continuous monitoring of cellular processes offers distinct advantages over traditional endpoint assays. A comprehensive representation of the changes occurring in live cells over the entire length of an experiment provides information about the biological status of the cell and informs decisions about the timing of treatments or the use of other functional endpoint assays. We describe a homogeneous, nonlytic, bioluminescent assay that measures cell viability in real time. This time-dependent measurement allowed us to monitor cell health for 72 h from the same test samples, distinguish differential cell growth, and investigate drug mechanism of action by analyzing time- and dose-dependent drug effects. The real-time measurements also allowed us to detect cell death immediately (>

SUBMITTER: Duellman SJ 

PROVIDER: S-EPMC4605357 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature