Cell-type specific metabolic profiling of Arabidopsis thaliana protoplasts as a tool for plant systems biology.

ABSTRACT: Flow cytometry combined with cell sorting of protoplasts has previously been used successfully for transcript profiling of the Arabidopsis thaliana root. We have developed the technique further, and in this paper we present a robust and reliable method for metabolite profiling in specific cell types isolated from Arabidopsis roots. The method uses a combination of fluorescence-activated cell sorting and gas chromatography-time of flight-mass spectrometry analysis. Cortical and endodermal cells from the green fluorescent protein (GFP)-expressing enhancer trap line J0571 were analysed and compared with non-GFP-expressing cells and intact root tissue. Of the metabolites identified, several showed significant differences in concentration between cell types. Multivariate statistic

SUBMITTER: Petersson SV 

PROVIDER: S-EPMC4605972 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature