Monoclonal antibodies indicate low-abundance links between heteroxylan and other glycans of plant cell walls.

ABSTRACT:

Main conclusion

The derivation of two sensitive monoclonal antibodies directed to heteroxylan cell wall polysaccharide preparations has allowed the identification of potential inter-linkages between xylan and pectin in potato tuber cell walls and also between xylan and arabinogalactan-proteins in oat grain cell walls. Plant cell walls are complex composites of structurally distinct glycans that are poorly understood in terms of both in muro inter-linkages and developmental functions. Monoclonal antibodies (MAbs) are versatile tools that can detect cell wall glycans with high sensitivity through the specific recognition of oligosaccharide structures. The isolation of two novel MAbs, LM27 and LM28, directed to heteroxylan, subsequent to immunisation with a potato cell wall fraction e

SUBMITTER: Cornuault V 

PROVIDER: S-EPMC4605975 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature