Metabolomics profiling of pre-and post-anesthesia plasma samples of colorectal patients obtained via Ficoll separation.

ABSTRACT: ¹H NMR spectroscopy was used to investigate the metabolic consequences of general anesthesia in the plasma of two groups of patients with diagnosis for non-metastatic colorectal cancer and metastatic colorectal cancer with liver-metastasis, respectively. Patients were treated with etomidate or propofol, two frequently used sedation agents. Plasma samples were obtained via Ficoll separation. Here, we demonstrated that this procedure introduces a number of limitations for NMR-based metabolomics studies, due to the appearance of spurious signals. Nevertheless, the comparison of the ¹H NMR metabolomic profiles of patients treated with etomidate or propofol at equipotent dose ranges was still feasible and proved that both agents significantly decrease the plasma levels of

SUBMITTER: Ghini V 

PROVIDER: S-EPMC4605980 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature