LC-MS-based absolute metabolite quantification: application to metabolic flux measurement in trypanosomes.

ABSTRACT: Human African trypanosomiasis is a neglected tropical disease caused by the protozoan parasite, Trypanosoma brucei. In the mammalian bloodstream, the trypanosome's metabolism differs significantly from that of its host. For example, the parasite relies exclusively on glycolysis for energy source. Recently, computational and mathematical models of trypanosome metabolism have been generated to assist in understanding the parasite metabolism with the aim of facilitating drug development. Optimisation of these models requires quantitative information, including metabolite concentrations and/or metabolic fluxes that have been hitherto unavailable on a large scale. Here, we have implemented an LC-MS-based method that allows large scale quantification of metabolite levels by using U-¹

SUBMITTER: Kim DH 

PROVIDER: S-EPMC4605981 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature