Improvement of glucaric acid production in E. coli via dynamic control of metabolic fluxes.

ABSTRACT: D-glucaric acid can be used as a building block for biopolymers as well as in the formulation of detergents and corrosion inhibitors. A biosynthetic route for production in E. coli has been developed (Moon et al., 2009), but previous work with the glucaric acid pathway has indicated that competition with endogenous metabolism may limit carbon flux into the pathway. Our group has recently developed an E. coli strain where phosphofructokinase (Pfk) activity can be dynamically controlled and demonstrated its use for improving yields and titers of the glucaric acid precursor myo-inositol on glucose minimal medium. In this work, we have explored the further applicability of this strain for glucaric acid production in a supplemented medium more relevant for scale-up studies,

SUBMITTER: Reizman IM 

PROVIDER: S-EPMC4606470 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature