Use of aminoglycoside 3' adenyltransferase as a selection marker for Chlamydia trachomatis intron-mutagenesis and in vivo intron stability.

ABSTRACT:

Background

C hlamydia spp. are obligate, intracellular bacteria that infect humans and animals. Research on these important pathogens has been hindered due to a paucity of genetic tools. We recently adapted a group II intron (GII) mutagenesis platform for creation of ampicillin-selectable gene insertions in C. trachomatis L2. The aims of this study were: (1) to assess the stability of the intron-insertion in an in vivo infection model to gauge the efficacy of this genetic tool for long term animal studies and (2) to expand upon the utility of the method by validating a second selection marker (aadA, conferring spectinomycin resistance) for mutant construction.

Results

Intron stability was assessed using a mouse vaginal tract infection model with a C. trachomatis L2 434/Bu in

SUBMITTER: Lowden NM 

PROVIDER: S-EPMC4606545 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature