Conserved Cis-Regulatory Modules Control Robustness in Msx1 Expression at Single-Cell Resolution.

ABSTRACT: The process of transcription is highly stochastic leading to cell-to-cell variations and noise in gene expression levels. However, key essential genes have to be precisely expressed at the correct amount and time to ensure proper cellular development and function. Studies in yeast and bacterial systems have shown that gene expression noise decreases as mean expression levels increase, a relationship that is controlled by promoter DNA sequence. However, the function of distal cis-regulatory modules (CRMs), an evolutionary novelty of metazoans, in controlling transcriptional robustness and variability is poorly understood. In this study, we used live cell imaging of transfected reporters combined with a mathematical modelling and statistical inference scheme to quantify the function of conse

SUBMITTER: Vance KW 

PROVIDER: S-EPMC4607535 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature