Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum.

ABSTRACT: OBJECTIVE:Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths. Novel serum biomarkers are required to increase the sensitivity and specificity of serum screening for early HCC diagnosis. This study employed a quantitative proteomic strategy to analyze the differential expression of serum glycoproteins between HCC and normal control serum samples. METHODS:Lectin affinity chromatography (LAC) was used to enrich glycoproteins from the serum samples. Quantitative mass spectrometric analysis combined with stable isotope dimethyl labeling and 2D liquid chromatography (LC) separations were performed to examine the differential levels of the detected proteins between HCC and control serum samples. Western blot was used to analyze the differential expression levels of the thr

SUBMITTER: Gao HJ 

PROVIDER: S-EPMC4607824 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature