Mass Spectrometric and Spectrofluorometric Studies of the Interaction of Aristolochic Acids with Proteins.

ABSTRACT: Aristolochic acid (AA) is a potent carcinogen and nephrotoxin and is associated with the development of "Chinese herb nephropathy" and Balkan endemic nephropathy. Despite decades of research, the specific mechanism of the observed nephrotoxicity has remained elusive and the potential effects on proteins due to the observed toxicity of AA are not well-understood. To better understand the pharmacotoxicological features of AA, we investigated the non-covalent interactions of AA with proteins. The protein-binding properties of AA with bovine serum albumin (BSA) and lysozyme were characterized using spectrofluorometric and mass spectrometric (MS) techniques. Moreover, the protein-AA complexes were clearly identified by high-resolution MS analyses. To the best of our knowledge, this is the first

SUBMITTER: Li W 

PROVIDER: S-EPMC4608009 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature