Genetic and nutrient modulation of acetyl-CoA levels in Synechocystis for n-butanol production.

ABSTRACT:

Background

There is a strong interest in using photosynthetic cyanobacteria as production hosts for biofuels and chemicals. Recent work has shown the benefit of pathway engineering, enzyme tolerance, and co-factor usage for improving yields of fermentation products.

Results

An n-butanol pathway was inserted into a Synechocystis mutant deficient in polyhydroxybutyrate synthesis. We found that nitrogen starvation increased specific butanol productivity up to threefold, but cessation of cell growth limited total n-butanol titers. Metabolite profiling showed that acetyl-CoA increased twofold during nitrogen starvation. Introduction of a phosphoketolase increased acetyl-CoA levels sixfold at nitrogen replete conditions and increased butanol titers from 22 to 37 mg/L at day 8. Flu

SUBMITTER: Anfelt J 

PROVIDER: S-EPMC4609045 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature