Derivation of induced pluripotent stem cells from orangutan skin fibroblasts.

ABSTRACT:

Background

Orangutans are an endangered species whose natural habitats are restricted to the Southeast Asian islands of Borneo and Sumatra. Along with the African great apes, orangutans are among the closest living relatives to humans. For potential species conservation and functional genomics studies, we derived induced pluripotent stem cells (iPSCs) from cryopreserved somatic cells obtained from captive orangutans.

Results

Primary skin fibroblasts from two Sumatran orangutans were transduced with retroviral vectors expressing the human OCT4, SOX2, KLF4, and c-MYC factors. Candidate orangutan iPSCs were characterized by global gene expression and DNA copy number analysis. All were consistent with pluripotency and provided no evidence of large genomic insertions or deletions

SUBMITTER: Ramaswamy K 

PROVIDER: S-EPMC4609060 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature