Changes in Lolium perenne transcriptome during cold acclimation in two genotypes adapted to different climatic conditions.

ABSTRACT:

Background

Activation of numerous protective mechanisms during cold acclimation is important for the acquisition of freezing tolerance in perennial ryegrass (Lolium perenne L.). To elucidate the molecular mechanisms of cold acclimation in two genotypes ('Veyo' and 'Falster') of perennial ryegrass from distinct geographical origins, we performed transcriptome profiling during cold acclimation using RNA-Seq.

Methods

We cold-acclimated plants from both genotypes in controlled conditions for a period of 17 days and isolated Total RNA at various time points for high throughput sequencing using Illumina technology. RNA-seq reads were aligned to genotype specific references to identify transcripts with significant changes in expression during cold acclimation.

Results

The ge

SUBMITTER: Abeynayake SW 

PROVIDER: S-EPMC4609083 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature