A GntR family transcription factor positively regulates mycobacterial isoniazid resistance by controlling the expression of a putative permease.

ABSTRACT:

Background

Bacteria use transcriptional regulation to respond to environmental stresses. Specifically, exposure to antibacterial drugs is deemed to be an atypical stress, and altering transcriptional regulation in response to such stress can increase bacterial drug resistance. However, only a few transcription factors that regulate drug resistance have been reported.

Results

In the present study, a GntR family transcription factor, encoded by the MSMEG_0535 (Ms0535) gene, was shown to be an isoniazid (INH) resistance regulator in Mycobacterium smegmatis. When the Ms0535 gene was overexpressed, cells showed a significant increase in INH resistance. First, the interaction between Ms0535 and its own promoter was determined, and a conserved 26-bp palindromic DNA binding motif wa

SUBMITTER: Hu J 

PROVIDER: S-EPMC4609117 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature