Identification of two mutations increasing the methanol tolerance of Corynebacterium glutamicum.

ABSTRACT:

Background

Methanol is present in most ecosystems and may also occur in industrial applications, e.g. as an impurity of carbon sources such as technical glycerol. Methanol often inhibits growth of bacteria, thus, methanol tolerance may limit fermentative production processes.

Results

The methanol tolerance of the amino acid producing soil bacterium Corynebacterium glutamicum was improved by experimental evolution in the presence of methanol. The resulting strain Tol1 exhibited significantly increased growth rates in the presence of up to 1 M methanol. However, neither transcriptional changes nor increased enzyme activities of the linear methanol oxidation pathway were observed, which was in accordance with the finding that tolerance to the downstream metabolites formaldehyde

SUBMITTER: Leßmeier L 

PROVIDER: S-EPMC4609165 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature