Detection of Staphylococcus Enterotoxin B (SEB) Using an Immunochromatographic Test Strip.

ABSTRACT: BACKGROUND:Staphylococcus aureus is one of the most important microorganisms that causes various human diseases by secreting virulence factors known as staphylococcal super antigens (SAgs). Staphylococcal Enterotoxin B (SEB) is a bacterial antigen that is responsible for food poisoning in humans. Among SEB detection methods, a lateral flow device (LFD) is ideal for rapid immunochromatographic tests because it is easy to use, requires minimal time to produce results, and does not require personnel training. OBJECTIVES:In our laboratory, the production of an immunochromatographic test strip, for the detection of SEB using a sandwich assay and a competitive method, was described; the test can detect SEB with high sensitivity. MATERIALS AND METHODS:The strip assays were compared with PCR, a va

SUBMITTER: Gholamzad M 

PROVIDER: S-EPMC4609312 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature