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A Pyrosequencing Assay for the Quantitative Methylation Analysis of GALR1 in Endometrial Samples: Preliminary Results.

ABSTRACT: Endometrial cancer is the most common malignancy of the female genital tract while aberrant DNA methylation seems to play a critical role in endometrial carcinogenesis. Galanin's expression has been involved in many cancers. We developed a new pyrosequencing assay that quantifies DNA methylation of galanin's receptor-1 (GALR1). In this study, the preliminary results indicate that pyrosequencing methylation analysis of GALR1 promoter can be a useful ancillary marker to cytology as the histological status can successfully predict. This marker has the potential to lead towards better management of women with endometrial lesions and eventually reduce unnecessary interventions. In addition it can provide early warning for women with negative cytological result.

SUBMITTER: Kottaridi C 

PROVIDER: S-EPMC4609388 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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A Pyrosequencing Assay for the Quantitative Methylation Analysis of GALR1 in Endometrial Samples: Preliminary Results.

Kottaridi Christine C   Koureas Nikolaos N   Margari Niki N   Terzakis Emmanouil E   Bilirakis Evripidis E   Pappas Asimakis A   Chrelias Charalampos C   Spathis Aris A   Aga Evangelia E   Pouliakis Abraham A   Panayiotides Ioannis I   Karakitsos Petros P  

BioMed research international 20151004

Endometrial cancer is the most common malignancy of the female genital tract while aberrant DNA methylation seems to play a critical role in endometrial carcinogenesis. Galanin's expression has been involved in many cancers. We developed a new pyrosequencing assay that quantifies DNA methylation of galanin's receptor-1 (GALR1). In this study, the preliminary results indicate that pyrosequencing methylation analysis of GALR1 promoter can be a useful ancillary marker to cytology as the histologica  ...[more]

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