Coexpression of TIGIT and FCRL3 identifies Helios+ human memory regulatory T cells.

ABSTRACT: Two distinct subsets of CD4(+)Foxp3(+) regulatory T (Treg) cells have been described based on the differential expression of Helios, a transcription factor of the Ikaros family. Efforts to understand the origin and biological roles of these Treg populations in regulating immune responses have, however, been hindered by the lack of reliable surface markers to distinguish and isolate them for subsequent functional studies. Using a single-cell cloning strategy coupled with microarray analysis of different Treg functional subsets in humans, we identify the mRNA and protein expression of TIGIT and FCRL3 as a novel surface marker combination that distinguishes Helios(+)FOXP3(+) from Helios(-)FOXP3(+) memory cells. Unlike conventional markers that are modulated on conventional T cells upon activa

SUBMITTER: Bin Dhuban K 

PROVIDER: S-EPMC4610024 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature