Universal Capacitance Model for Real-Time Biomass in Cell Culture.

ABSTRACT: : Capacitance probes have the potential to revolutionize bioprocess control due to their safe and robust use and ability to detect even the smallest capacitors in the form of biological cells. Several techniques have evolved to model biomass statistically, however, there are problems with model transfer between cell lines and process conditions. Errors of transferred models in the declining phase of the culture range for linear models around +100% or worse, causing unnecessary delays with test runs during bioprocess development. The goal of this work was to develop one single universal model which can be adapted by considering a potentially mechanistic factor to estimate biomass in yet untested clones and scales. The novelty of this work is a methodology to select sensitive frequencies to

SUBMITTER: Konakovsky V 

PROVIDER: S-EPMC4610510 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature