High-Throughput Sequencing-The Key to Rapid Biodiversity Assessment of Marine Metazoa?

ABSTRACT: The applications of traditional morphological and molecular methods for species identification are greatly restricted by processing speed and on a regional or greater scale are generally considered unfeasible. In this context, high-throughput sequencing, or metagenetics, has been proposed as an efficient tool to document biodiversity. Here we evaluated the effectiveness of 454 pyrosequencing in marine metazoan community analysis using the 18S rDNA: V1-V2 region. Multiplex pyrosequencing of the V1-V2 region was used to analyze two pooled samples of DNA, one comprising 118 and the other 37 morphologically identified species, and one natural sample taken directly from a North Sea zooplankton community. A DNA reference library comprising all species represented in the pooled samples was create

SUBMITTER: Mohrbeck I 

PROVIDER: S-EPMC4610693 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature