Virome Capture Sequencing Enables Sensitive Viral Diagnosis and Comprehensive Virome Analysis.

ABSTRACT: Insensitivity and technical complexity have impeded the implementation of high-throughput nucleic acid sequencing in differential diagnosis of viral infections in clinical laboratories. Here, we describe the development of a virome capture sequencing platform for vertebrate viruses (VirCapSeq-VERT) that increases the sensitivity of sequence-based virus detection and characterization. The system uses ~2 million probes that cover the genomes of members of the 207 viral taxa known to infect vertebrates, including humans. A biotinylated oligonucleotide library was synthesized on the NimbleGen cleavable array platform and used for solution-based capture of viral nucleic acids present in complex samples containing variable proportions of viral and host nucleic acids. The use of VirCapSeq-VERT re

SUBMITTER: Briese T 

PROVIDER: S-EPMC4611031 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature