A novel hepatitis B virus genotyping system by using restriction fragment length polymorphism patterns of S gene amplicons.

ABSTRACT:

Aim

Traditional hepatitis B virus (HBV) genotyping methods using restriction fragment length polymorphism (RFLP) can reliably identify genotypes A to F. As HBV genotypes G and H have been recently identified, this study was to establish an accurate and simple genotyping method for all eight HBV genotypes (A to H).

Methods

Two hundred and forty HBV small S sequences obtained from GeneBank were analysed for restriction enzyme sites that would be genotype-specific. Restriction patterns following digestion with restriction enzymes BsrI, StyI, DpnI, HpaII, and EaeI, were determined to identify all eight HBV genotypes. Mixed genotype infections were confirmed by cloning and further RFLP analysis.

Results

The new genotyping method could identify HBV genotypes A to H. Genotyp

SUBMITTER: Zeng GB 

PROVIDER: S-EPMC4611256 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature