ShRNA-mediated gene silencing of beta-catenin inhibits growth of human colon cancer cells.

ABSTRACT:

Aim

To observe the gene silencing mediated by the specific shRNA targeted against beta-catenin and its effect on cell proliferation and cycle distribution in the human colon cancer cell line Colo205.

Methods

Two shRNA plasmid vectors against beta-catenin were constructed and transfected into Colo205 cells with Lipofectamine2000. The down-regulations of beta-catenin, c-myc and cyclinD1 expressions were detected by RT-PCR and western blot analysis. The cell proliferation inhibitions were determined by MTT assay and soft agar colony formation assay. The effect of these two beta-catenin shRNAs on cell cycle distribution and apoptosis was examined by flow cytometry.

Results

These two shRNA vectors targeted against beta-catenin efficiently suppressed the expression of beta-

SUBMITTER: Huang WS 

PROVIDER: S-EPMC4611300 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature