Single-cell genomics-based analysis of virus-host interactions in marine surface bacterioplankton.

ABSTRACT: Viral infections dynamically alter the composition and metabolic potential of marine microbial communities and the evolutionary trajectories of host populations with resulting feedback on biogeochemical cycles. It is quite possible that all microbial populations in the ocean are impacted by viral infections. Our knowledge of virus-host relationships, however, has been limited to a minute fraction of cultivated host groups. Here, we utilized single-cell sequencing to obtain genomic blueprints of viruses inside or attached to individual bacterial and archaeal cells captured in their native environment, circumventing the need for host and virus cultivation. A combination of comparative genomics, metagenomic fragment recruitment, sequence anomalies and irregularities in sequence coverage depth

SUBMITTER: Labonte JM 

PROVIDER: S-EPMC4611503 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature