New roles for DNA cytosine modification, eRNA, anchors, and superanchors in developing B cell progenitors.

ABSTRACT: B-cell fate is orchestrated by a series of well-characterized developmental regulators. Here, we found that the onset of B-cell development was accompanied by large-scale changes in DNA cytosine modifications associated with promoters, enhancers, and anchors. These changes were tightly linked to alterations in transcription factor occupancy and nascent RNA (eRNA) transcription. We found that the prepro-B to the pro-B-cell transition was associated with a global exchange of DNA cytosine modifications for polycomb-mediated repression at CpG islands. Hypomethylated regions were found exclusively in the active/permissive compartment of the nucleus and were predominantly associated with regulatory elements or anchors that orchestrate the folding patterns of the genome. We identified superanchor

SUBMITTER: Benner C 

PROVIDER: S-EPMC4611620 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature