Isolation and analysis of a novel gene over-expressed during liver regeneration.

ABSTRACT: AIM:To isolate and analyze a novel gene over-expressed during liver regeneration. METHODS:Total RNA of regenerating liver was extracted from liver tissue after 0-4-36-36-36 hr short interval successive partial hepatectomy (SISPH). Reverse transcription-polymerase chain reaction was used to synthesize double strand cDNA, after the tissue was digested by proteinase K and Sfi A/B. The double-strand cDNA was ligated to lambdaTriplEx2. lambdaphage packaging reaction was performed and E. coli XL1-Blue was infected for titering and amplifying. One expressed sequence tag was probed by Dig and phage in situ hybridization was carried out to isolate positive clones. Positive recombinant lambdaTriplEx2 was converted to the corresponding pTriplEx2, and bioinformatics was used to analyze full-length cDN

SUBMITTER: Li YC 

PROVIDER: S-EPMC4611801 | biostudies-literature | 2003 Jun

REPOSITORIES: biostudies-literature