Molecular evolution of hepatitis A virus in a human diploid cell line.

ABSTRACT:

Aim

To investigate the hotspots, direction, and the time course of evolution of hepatitis A virus in the process of consecutive cell culture passage in human KMB17 diploid cells.

Methods

Wild type hepatitis A virus H2w was serially propagated in KMB17 cells until passage 30, and the full-length genomes of H2w and its six chosen progenies were determined by directly sequencing RT-PCR products amplified from viral genomic RNA. Alignment comparison of sequences from H2w with its six progenies and phylogenetic analysis of the whole VP1 region from H2w, progenies of H2w, and other cell culture adapted hepatitis A virus were then carried out to obtain data on the molecular evolution of hepatitis A virus in the process of consecutive passage in KMB17 cells.

Results

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SUBMITTER: Tang CH 

PROVIDER: S-EPMC4611841 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature