High-throughput nucleotide sequence analysis of diverse bacterial communities in leachates of decomposing pig carcasses.

ABSTRACT: The leachate generated by the decomposition of animal carcass has been implicated as an environmental contaminant surrounding the burial site. High-throughput nucleotide sequencing was conducted to investigate the bacterial communities in leachates from the decomposition of pig carcasses. We acquired 51,230 reads from six different samples (1, 2, 3, 4, 6 and 14 week-old carcasses) and found that sequences representing the phylum Firmicutes predominated. The diversity of bacterial 16S rRNA gene sequences in the leachate was the highest at 6 weeks, in contrast to those at 2 and 14 weeks. The relative abundance of Firmicutes was reduced, while the proportion of Bacteroidetes and Proteobacteria increased from 3-6 weeks. The representation of phyla was restored after 14 weeks. However, the comm

SUBMITTER: Yang SH 

PROVIDER: S-EPMC4612598 | biostudies-literature | 2015 Jul-Sep

REPOSITORIES: biostudies-literature