Expression of purinergic receptor P2Y4 in Schwann cell following nerve regeneration.

ABSTRACT:

Objective

Emerging evidences suggested an important role of purinergic receptor P2Y4 in nerve system. The present study aims to investigate the localization and possible function of P2Y4 receptor in recurrent laryngeal nerve (RLN) following regeneration.

Methods

Right RLN of fifty Sprague-Dawley rats was cut and immediately repaired with PLGA/chitosan nerve conduit. Immunofluorescence, real-time qPCR and Western blot were used to detect the expression alterations of P2Y4 receptor.

Results

Weak immunostaining signals of P2Y4 receptor were located on the plasmalemma of Schwann cell (SC) with regular arrangement of axons in normal RLN. On the post-injury 4th day, the sprouting axons regrowed along the degenerated SCs intensively expressing P2Y4 receptor. On the post-inju

SUBMITTER: Chen S 

PROVIDER: S-EPMC4612929 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature