MiR-29a promotes myocardial cell apoptosis induced by high glucose through down-regulating IGF-1.

ABSTRACT: This study was aimed to investigate the role of miR-29a in myocardial cell apoptosis induced by high glucose. Myocardial cells were cultured in normal (5.6 mmol/l) or high glucose medium (30 mmol/l). The apoptotic rate of myocardial cells was evaluated using flow cytometry. The mRNA levels of Bax, Bcl-2, miR-29a, and IGF-1 were determined using real-time quantitative PCR (RT-qPCR). The level of IGF-1 in the culture medium was analyzed using enzyme-linked immunosorbent assay (ELISA). The interaction sites between miR-29a and IGF-1 was analyzed using the the Targetscan program. The regulatory effect of miR-29a on the expression of IGF-1 was investigated using dual luciferase reporter system. The results showed that the expression of miR-29a and the Bax/Bcl-2 ratio in myocardial cells were si

SUBMITTER: Han C 

PROVIDER: S-EPMC4613106 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature