The regulation of mitochondrial transcription factor A (Tfam) expression during skeletal muscle cell differentiation.

ABSTRACT: The ATP demand required for muscle development is accommodated by elevations in mitochondrial biogenesis, through the co-ordinated activities of the nuclear and mitochondrial genomes. The most important transcriptional activator of the mitochondrial genome is mitochondrial transcription factor A (Tfam); however, the regulation of Tfam expression during muscle differentiation is not known. Thus, we measured Tfam mRNA levels, mRNA stability, protein expression and localization and Tfam transcription during the progression of muscle differentiation. Parallel 2-fold increases in Tfam protein and mRNA were observed, corresponding with 2-3-fold increases in mitochondrial content. Transcriptional activity of a 2051 bp promoter increased during this differentiation period and this was accompanied

SUBMITTER: Collu-Marchese M 

PROVIDER: S-EPMC4613705 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature