Computer-assisted quantification of motile and invasive capabilities of cancer cells.

ABSTRACT: High-throughput analysis of cancer cell dissemination and its control by extrinsic and intrinsic cellular factors is hampered by the lack of adequate and efficient analytical tools for quantifying cell motility. Oncology research would greatly benefit from such a methodology that allows to rapidly determine the motile behaviour of cancer cells under different environmental conditions, including inside three-dimensional matrices. We combined automated microscopy imaging of two- and three-dimensional cell cultures with computational image analysis into a single assay platform for studying cell dissemination in high-throughput. We have validated this new approach for medulloblastoma, a metastatic paediatric brain tumour, in combination with the activation of growth factor signalling pathways

SUBMITTER: Kumar KS 

PROVIDER: S-EPMC4614254 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature