Citrullination of myofilament proteins in heart failure.

ABSTRACT:

Aims

Citrullination, the post-translational conversion of arginine to citrulline by the enzyme family of peptidylarginine deiminases (PADs), is associated with several diseases, and specific citrullinated proteins have been shown to alter function while others act as auto-antigens. In this study, we identified citrullinated proteins in human myocardial samples, from healthy and heart failure patients, and determined several potential functional consequences. Further we investigated PAD isoform cell-specific expression in the heart.

Methods and results

A citrullination-targeted proteomic strategy using data-independent (SWATH) acquisition method was used to identify the modified cardiac proteins. Citrullinated-induced sarcomeric proteins were validated using two-dimensional g

SUBMITTER: Fert-Bober J 

PROVIDER: S-EPMC4614685 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature