Expression Profiling of Human Schlemm's Canal Endothelial Cells From Eyes With and Without Glaucoma.

ABSTRACT:

Purpose

Ocular hypertension is a major risk factor for glaucoma and the inner wall of Schlemm's canal (SC) endothelia participates in the regulation of aqueous humor outflow resistance. This study aimed to identify differentially expressed genes in primary cultures of SC cells from glaucoma patients.

Methods

This study examined SC samples from three glaucoma cases and four controls. Schlemm's canal cells were isolated from eight different postmortem human eyes. Total RNA was extracted, labeled, and hybridized to Illumina HumanWG-6 BeadChips containing probes for approximately 47,000 human transcripts. After extracting the data using Illumina GenomeStudio software, the data were normalized and analyzed using the R package limma in Bioconductor. Using Protein ANalysis THrough

SUBMITTER: Cai J 

PROVIDER: S-EPMC4614909 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature