A phenotypic screen identifies microtubule plus end assembly regulators that can function in mitotic spindle orientation.

ABSTRACT: Proper regulation of microtubule dynamics during mitosis is essential for faithful chromosome segregation. In fact, recently we discovered increased microtubule plus end assembly rates that are frequently observed in human cancer cells as an important mechanism leading to whole chromosome missegregation and chromosomal instability (CIN). However, the genetic alterations responsible for increased microtubule polymerization rates in cancer cells remain largely unknown. The identification of such lesions is hampered by the fact that determining dynamic parameters of microtubules usually involves analyses of living cells, which is technically difficult to perform in large-scale screening settings. Therefore, we sought to identify alternative options to systematically identify regulators of mic

SUBMITTER: Stolz A 

PROVIDER: S-EPMC4614948 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature