The role of short RNA loops in recognition of a single-hairpin exon derived from a mammalian-wide interspersed repeat.

ABSTRACT: Splice-site selection is controlled by secondary structure through sequestration or approximation of splicing signals in primary transcripts but the exact role of even the simplest and most prevalent structural motifs in exon recognition remains poorly understood. Here we took advantage of a single-hairpin exon that was activated in a mammalian-wide interspersed repeat (MIR) by a mutation stabilizing a terminal triloop, with splice sites positioned close to each other in a lower stem of the hairpin. We first show that the MIR exon inclusion in mRNA correlated inversely with hairpin stabilities. Employing a systematic manipulation of unpaired regions without altering splice-site configuration, we demonstrate a high correlation between exon inclusion of terminal tri- and tetraloop mutants an

SUBMITTER: Kralovicova J 

PROVIDER: S-EPMC4615370 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature