Preparation and characterization of polyclonal antibodies against ARL-1 protein.

ABSTRACT:

Aim

To prepare and characterize polyclonal antibodies against aldose reductase-like (ARL-1) protein.

Methods

ARL-1 gene was inserted into the E. coli expression vector pGEX-4T-1(His)(6)C and vector pQE-30. Recombinant ARL-1 proteins named ARL-(His)(6) and ARL-GST were expressed. They were purified by affinity chromatography. Sera from domestic rabbits immunized with ARL-(His) (6) were purified by CNBr-activated sepharose 4B coupled ARL-GST. Polyclonal antibodies were detected by Western blotting.

Results

Recombinant proteins of ARL-(His)(6) with molecular weight of 35.7 KD and ARL-GST with molecular weight of 60.8 KD were highly expressed. The expression levels of ARL-GST and ARL-(His)(6) were 15.1 % and 27.7 % among total bacteria proteins, respectively. They were so

SUBMITTER: Jin JF 

PROVIDER: S-EPMC4615481 | biostudies-literature | 2003 Jul

REPOSITORIES: biostudies-literature