Graphene oxide as a protein matrix: influence on protein biophysical properties.

ABSTRACT:

Background

This study provides fundamental information on the influence of graphene oxide (GO) nanosheets and glycans on protein catalytic activity, dynamics, and thermal stability. We provide evidence of protein stabilization by glycans and how this strategy could be implemented when GO nanosheets is used as protein immobilization matrix. A series of bioconjugates was constructed using two different strategies: adsorbing or covalently attaching native and glycosylated bilirubin oxidase (BOD) to GO.

Results

Bioconjugate formation was followed by FT-IR, zeta-potential, and X-ray photoelectron spectroscopy measurements. Enzyme kinetic parameters (k(m) and k(cat)) revealed that the substrate binding affinity was not affected by glycosylation and immobilization on GO, but the ra

SUBMITTER: Hernandez-Cancel G 

PROVIDER: S-EPMC4617716 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature