Integrated analysis of the local and systemic changes preceding the development of post-partum cytological endometritis.

ABSTRACT:

Background

The regulation of endometrial inflammation has important consequences for the resumption of bovine fertility postpartum. All cows experience bacterial influx into the uterus after calving; however a significant proportion fail to clear infection leading to the development of cytological endometritis (CE) and compromised fertility. We hypothesised that early immunological changes could not only act as potential prognostic biomarkers for the subsequent development of disease but also shed light on the pathogenesis of endometritis in the postpartum dairy cow.

Methods

Endometrial biopsy RNA was extracted from 15 cows at 7 and 21 days postpartum (DPP), using the Qiagen RNeasy(®) Plus Mini kit and quality determined using an Agilent 2100 bioanalyser. Disease status was

SUBMITTER: Foley C 

PROVIDER: S-EPMC4617749 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature