Cooperative target mRNA destabilization and translation inhibition by miR-58 microRNA family in C. elegans.

ABSTRACT: In animals, microRNAs frequently form families with related sequences. The functional relevance of miRNA families and the relative contribution of family members to target repression have remained, however, largely unexplored. Here, we used the Caenorhabditis elegans miR-58 miRNA family, composed primarily of the four highly abundant members miR-58.1, miR-80, miR-81, and miR-82, as a model to investigate the redundancy of miRNA family members and their impact on target expression in an in vivo setting. We found that miR-58 family members repress largely overlapping sets of targets in a predominantly additive fashion. Progressive deletions of miR-58 family members lead to cumulative up-regulation of target protein and RNA levels. Phenotypic defects could only be observed in the family quadr

SUBMITTER: Subasic D 

PROVIDER: S-EPMC4617964 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature