New global analysis of the microRNA transcriptome of primary tumors and lymph node metastases of papillary thyroid cancer.

ABSTRACT:

Background

Papillary Thyroid Cancer (PTC) is the most prevalent type of endocrine cancer. Its incidence has rapidly increased in recent decades but little is known regarding its complete microRNA transcriptome (miRNome). In addition, there is a need for molecular biomarkers allowing improved PTC diagnosis.

Methods

We performed small RNA deep-sequencing of 3 PTC, their matching normal tissues and lymph node metastases (LNM). We designed a new bioinformatics framework to handle each aspect of the miRNome: whole expression profiles, isomiRs distribution, non-templated additions distributions, RNA-editing or mutation. Results were validated experimentally by qRT-PCR on normal samples, tumors and LNM from 14 independent patients and in silico using the dataset from The Cancer Gen

SUBMITTER: Saiselet M 

PROVIDER: S-EPMC4618137 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature