EPR Spectroscopic Studies of [FeFe]-Hydrogenase Maturation.

ABSTRACT: Proton reduction and H₂ oxidation are key elementary reactions for solar fuel production. Hydrogenases interconvert H⁺ and H₂ with remarkable efficiency and have therefore received much attention in this context. For [FeFe]-hydrogenases, catalysis occurs at a unique cofactor called the H-cluster. In this article, we discuss ways in which EPR spectroscopy has elucidated aspects of the bioassembly of the H-cluster, with a focus on four case studies: EPR spectroscopic identification of a radical en route to the CO and CN^- ligands of the H-cluster, tracing ⁵⁷Fe from the maturase HydG into the H-cluster, characterization of the auxiliary Fe-S cluster in HydG, and isotopic labeling of the CN^- ligands of HydA for electronic

SUBMITTER: Suess DL 

PROVIDER: S-EPMC4618401 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature