Identification of novel and conserved microRNAs in Panax notoginseng roots by high-throughput sequencing.

ABSTRACT:

Background

MicroRNAs (miRNAs) are small, non-coding RNAs that are important regulators of gene expression, and play major roles in plant development and their response to the environment. Root extracts from Panax notoginseng contain triterpene saponins as their principal bioactive constituent, and demonstrate medicinal properties. To investigate the novel and conserved miRNAs in P. notoginseng, three small RNA libraries constructed from 1-, 2-, and 3-year-old roots in which root saponin levels vary underwent high-throughput sequencing.

Methods

P. notoginseng roots, purified from 1-, 2-, and 3-year-old roots, were extracted for RNA, respectively. Three small libraries were constructed and subjected to next generation sequencing.

Results

Sequencing of the three librarie

SUBMITTER: Wei R 

PROVIDER: S-EPMC4618736 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature