Genome-wide DNA methylome variation in two genetically distinct chicken lines using MethylC-seq.

ABSTRACT:

Background

DNA cytosine methylation is an important epigenetic modification that has significant effects on a variety of biological processes in animals. Avian species hold a crucial position in evolutionary history. In this study, we used whole-genome bisulfite sequencing (MethylC-seq) to generate single base methylation profiles of lungs in two genetically distinct and highly inbred chicken lines (Fayoumi and Leghorn) that differ in genetic resistance to multiple pathogens, and we explored the potential regulatory role of DNA methylation associated with immune response differences between the two chicken lines.

Methods

The MethylC-seq was used to generate single base DNA methylation profiles of Fayoumi and Leghorn birds. In addition, transcriptome profiling using RNA-seq f

SUBMITTER: Li J 

PROVIDER: S-EPMC4619007 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature