Identification of sensory hair-cell transcripts by thiouracil-tagging in zebrafish.

ABSTRACT:

Background

Sensory hair cells are exquisitely sensitive to mechanical stimuli and as such, are prone to damage and apoptosis during dissections or in vitro manipulations. Thiouracil (TU)-tagging is a noninvasive method to label cell type-specific transcripts in an intact organism, thereby meeting the challenge of how to analyze gene expression in hair cells without the need to sort cells. We adapted TU-tagging to zebrafish to identify novel transcripts expressed in the sensory hair cells of the developing acoustico-lateralis organs.

Methods

We created a transgenic line of zebrafish expressing the T.gondii uracil phospho-ribosyltransferase (UPRT) enzyme specifically in the hair cells of the inner ear and lateral line organ. RNA was labeled by exposing 3 days post-fertilizatio

SUBMITTER: Erickson T 

PROVIDER: S-EPMC4619078 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature