Histone acetylation regulates prostate ductal morphogenesis through a bone morphogenetic protein-dependent mechanism.

ABSTRACT:

Background

Epigenetic factors influence stem cell function and other developmental events but their role in prostate morphogenesis is not completely known. We tested the hypothesis that histone deacetylase (HDAC) activity is required for prostate morphogenesis.

Results

We identified the presence of class I nuclear HDACs in the mouse urogenital sinus (UGS) during prostate development and found that Hdac 2 mRNA abundance diminishes as development proceeds which is especially evident in prostatic epithelium. Blockade of HDACs with the inhibitor trichostatin A (TSA) decreased the number of prostatic buds formed in UGS explant cultures but not the number of buds undergoing branching morphogenesis. In the latter, TSA promoted an extensive branching phenotype that was reversed by e

SUBMITTER: Keil KP 

PROVIDER: S-EPMC4619142 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature