Detection of the HA-33 protein in botulinum neurotoxin type G complex by mass spectrometry.

ABSTRACT:

Background

The disease botulism is caused by intoxication with botulinum neurotoxins (BoNTs), extremely toxic proteins which cause paralysis. This neurotoxin is produced by some members of the Clostridium botulinum and closely related species, and is produced as a protein complex consisting of the neurotoxin and neurotoxin-associated proteins (NAPs). There are seven known serotypes of BoNT, A-G, and the composition of the NAPs can differ between these serotypes. It was previously published that the BoNT/G complex consisted of BoNT/G, nontoxic-nonhemagglutinin (NTNH), Hemagglutinin 70 (HA-70), and HA-17, but that HA-33, a component of the protein complex of other serotypes of BoNT, was not found.

Methods

Components of the BoNT/G complex were first separated by SDS-PAGE, and b

SUBMITTER: Kalb SR 

PROVIDER: S-EPMC4619279 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature