Molt-dependent transcriptomic analysis of cement proteins in the barnacle Amphibalanus amphitrite.

ABSTRACT:

Background

A complete understanding of barnacle adhesion remains elusive as the process occurs within and beneath the confines of a rigid calcified shell. Barnacle cement is mainly proteinaceous and several individual proteins have been identified in the hardened cement at the barnacle-substrate interface. Little is known about the molt- and tissue-specific expression of cement protein genes but could offer valuable insight into the complex multi-step processes of barnacle growth and adhesion.

Methods

The main body and sub-mantle tissue of the barnacle Amphibalanus amphitrite (basionym Balanus amphitrite) were collected in pre- and post-molt stages. RNA-seq technology was used to analyze the transcriptome for differential gene expression at these two stages and liquid chroma

SUBMITTER: Wang Z 

PROVIDER: S-EPMC4619306 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature