Cloning and characterization of the pepper CaPAO gene for defense responses to salt-induced leaf senescence.

ABSTRACT:

Background

Pheophorbide a oxygenase (PAO) is an important enzyme in the chlorophyll catabolism pathway and is involved in leaf senescence. It opens the porphyrin macrocycle of pheophorbide a and finally forms the primary fluorescent chlorophyll catabolite. Previous studies have demonstrated the function of PAO during cell death. However, the characterizaton of PAO during leaf senescence induced by environmental factors is not well understood.

Methods

Homology-based cloning and RACE techniques were used to obtain the full-length cDNA of the CaPAO gene. CaPAO expression was determined by quantitative real-time PCR. Function of CaPAO gene were studied using virus-induced gene silencing and transgenic techniques with tobacco plants (Nicotiana tabacum).

Results

A novel PAO

SUBMITTER: Xiao HJ 

PROVIDER: S-EPMC4619409 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature