Transcriptome analysis of Brassica napus pod using RNA-Seq and identification of lipid-related candidate genes.

ABSTRACT:

Background

Brassica napus is an important oilseed crop. Dissection of the genetic architecture underlying oil-related biological processes will greatly facilitates the genetic improvement of rapeseed. The differential gene expression during pod development offers a snapshot on the genes responsible for oil accumulation in. To identify candidate genes in the linkage peaks reported previously, we used RNA sequencing (RNA-Seq) technology to analyze the pod transcriptomes of German cultivar Sollux and Chinese inbred line Gaoyou.

Methods

The RNA samples were collected for RNA-Seq at 5-7, 15-17 and 25-27 days after flowering (DAF). Bioinformatics analysis was performed to investigate differentially expressed genes (DEGs). Gene annotation analysis was integrated with QTL mapping an

SUBMITTER: Xu HM 

PROVIDER: S-EPMC4619414 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature